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Figure 5d. Different transfer function settings alter the representation of the lumen. (a, b) Volume-rendered images created without shading at low opacity (a) and high opacity (b) show accentuated vessel boundaries. (c) On a volume-rendered image created with shading, the 3D impression is improved but edge definition is reduced. (The transfer functions in b and c are identical.) (d) Volume-rendered image created with the transfer function shifted toward higher Hounsfield unit values results in reduced caliber of the visualized vessels.